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[[_TOC_]]
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# Preface
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Herein are a few example experiments which decribe how to design experiments in Library Studio
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# Creating a transfer experiment between two plates with defined well coordinates
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This guide will show you how to create a simple experiment for the HTE platform using the GUI interface in Library Studio. The experiment in question is transferring 100 uL of liquid (water) from wells B1 to B3 (from now on B1:B3) from a source microplate ("Mother") to wells B4 to B6 in a target microplate ("Daughter").
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1. Start **Library Studio**
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2. From the **Tools** tab in the menu bar, click **Add New Library**, or click the grid symbol shown below: {width="456" height="65"}
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3. Create a name for the plate. In this example, we will name the plate "Mother". Set the well parameters for this microplate. In this example, we will set **Rows** to **4** and **Columns** to **6**. Click **OK** when you are done.
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4. Repeat the step above, but name this plate "Daughter"
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5. In the **Chemicals** tab (first to the left on the bottom menu), enter the **Name** of the chemical. In this case we will call it "Water"
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{width=371 height=61}
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(One option is to click the "Search Chemical Inventory" button, select "Name" under Field and then type "Water" under "Value", click "Search", which yields water. Check the box, then click "Add to Sources")
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6. In the sidepane, click "Water". Then highlight the wells B1:B3 in the "Mother" plate. In the drop-down menu "Map", choose "Exists". Choose "Water" for "Source", then "ul" for "Dispense Units". Click OK.
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7. You should now have question marks inside your highlighted cells. We will now define the volumes in the wells. Click "Formula" in the sidepane, then highlight the wells you want to select. Select ul for "Unit", then type in the dialoge box: ""water" = 200" (remember to wrap the chemical name inside quotation marks!). Click OK. The cells should now have the same colour as "Water" in the left pane.
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8. We will now define how and where the source wells should be transferred to. Click "Mother" on the sidepane, then select the cell range B1:B3 in the Mother plate. Then, select the cell range B4:B6 in the daughter plate (Notice how the selection highlight changes between the two selections). Define the Map as "Uniform", then make sure the the Dispense ane Design unit is set to "ul". Set "Value" to 200. You will notice that the highlighted cells will show the volume. By selecting "Discrete Amounts" in "Map", you can also define individual volumes per well.
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Click OK. |
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